Protein Labeling Reagents
Aat Bioquest ReadiLink™ Rapid mFluor™ Blue 570 Antibody Labeling Kit *Microscale Optimized for Labeling 50 µg Antibody Per Reaction*
AAT Bioquest's mFluor™ dyes are developed for flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm).
Biotium Mix-n-Stain™ CF405M Small Ligand Labeling Kit
Mix-n-Stain™ Small Ligand Labeling Kits are designed for rapid labeling of low molecular weight (MW 150 -5000) ligands without a purification step. Suitable ligands or substrates include SNAP-tag, CLIP-tag™ and HaloTag ligand derivatives that have an aliphatic amine. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF405M dye has excitation/emission at 408/452 nm. It is suitable for labeling ligands for cell surface staining. Note: Conjugates of blue fluorescent dyes are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Biotium 5-(AND-6)-CARBOXYTetramethylrhodamine SU
5(6)-TAMRA SE (full name: 5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester (mixed isomers)) is the amine reactive form of TAMRA, one of the most commonly used red fluorescent dyes. The succinimidyl ester reacts readily with primary or secondary amines under mild conditions. Properties: Ex/Em (MeOH) = 540/565 nm; e = 95,000; Red solid soluble in DMF or DMSO; Store at -20°C and protect from light; C 29 H 25 N 3 O 7; MW: 527.5; [150408-83-6]
Biotium Lucifer Yellow CH Potassium Salt
Lucifer Yellow CH potassium salt (LY CH potassium salt) is a widely used polar molecular tracer for studying neuronal morphology. The fluorescent molecule contains a carbohydrazide that allow the molecule to be aldehyde-fixable. Properties: Ex/ Em = 428/536 nm; Yellow solid soluble in water; Store at 4°C and protect from light; C13H9K2N5O9S2; MW: 522; [71206-95-6]
Biotium Mix-n-Stain™ CF640R Antibody Labeling Kit, 1x(50-100ug) labeling
Mix-n-Stain™ Antibody Labeling Kits are a revolutionary antibody labeling technology that allows you to label your antibody with one of our superior CF dyes in 30 minutes without a purification step. The labeling procedure tolerates many common buffer components and antibody stabilizers. Choose from kit sizes to label 5-20 ug, 20-50 ug, or 50-100 ug of your antibody. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red fluorescent CF640R dye has excitation/emission at 642/662 nm.
STEMCELL Technologies GloCell™ Fixable Viability Dye Red 780, Size: 500 Tests
GloCell™ Fixable Viability Dye Red 780 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Red 780 is excited by the red laser at 633 nm and it has a fluorescence emission maximum of 780 nm that can be detected using the 780/60 band pass filter used to detect APC-Cy7.
STEMCELL Technologies GloCell™ Fixable Viability Dye Violet 510, Size: 100 Tests
GloCell™ Fixable Viability Dye Violet 510 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.GloCell™ Fixable Viability Dye Violet 510 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 510 nm that can be detected using the 525/50 band pass filter used to detect AmCyan.
![Aat Bioquest Cyanine 3 monosuccinimidyl ester [equivalent to Cy3® NHS ester]](https://assets.fishersci.com/TFS-Assets/CCG/product-images/501952751.JPG-250.jpg)
Aat Bioquest Cyanine 3 monosuccinimidyl ester [equivalent to Cy3® NHS ester]
A variety of cyanine 3 (Cy3®) dyes has been used to label biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. They are widely used for labeling peptides, proteins and oligos etc.
Aat Bioquest iFluor™ 532 succinimidyl ester
AAT Bioquest's iFluor™ dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins.
![Aat Bioquest Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3*](https://assets.fishersci.com/TFS-Assets/CCG/product-images/501953129.JPG-250.jpg)
Aat Bioquest Tide Fluor™ 3 maleimide [TF3 maleimide] *Superior replacement for Cy3*
Tide Fluor™ 3 (TF3) family has the spectral properties essentially identical to those of Cy3. Compared to Cy3 probes TF3 family has much stronger fluorescence and higher photostability.
Aat Bioquest 6-TAMRA cadaverine
6-TAMRA cadaverine is a building block for developing fluoresceinated bioconjugates. It is also a fluorescent transglutaminase substrate to label proteins by transaminidation.
Bachem Mca-(Asn670,Leu671)-Amyloid Beta/A4 Protein Precursor770 (667-676)-Lys(Dnp)-Arg-Arg amide Trifluoroacetate
Mca-(Asn670,Leu671)-Amyloid Beta/A4 Protein Precursor770 (667-676)-Lys(Dnp)-Arg-Arg amide Trifluoroacetate, Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys(Dnp)-Arg-Arg-NH2, 438625-61-7, C86H125N27O29, Mr 2001.10, 1mg. Mca-(Asn670,Leu671)-APP770 (667-676)-Lys(Dnp)-Arg-Arg amide. This fluorescent (FRET) peptide substrate contains the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) b-secretase cleavage site. Mca-SEVNLDAEFRK(Dnp)RR-amide has been used for assaying b-secretase-like activity of thimet oligopeptidase (TOP, EC 3.4.24.15). The results suggested that TOP is a potential b-secretase candidate and is involved in the processing of APP in vivo.
Biotium Phalloidin, CF405M, 300 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Blue fluorescent CF405M has excitation/emission at 408/452 nm.
Biotium Phalloidin, CF680, 50 U
Phalloidin conjugates are actin filament stains for fixed and permeabilized cells. CF dyes are Biotium's line of next-generation fluorescent dyes with advantages in brightness, photostability, and conjugate specificity compared to other fluorescent dyes. Far-red/ near-IR fluorescent CF680 has excitation/emission at 681/698 nm.